Temperature sensitivity of mineral N transformation rates, and heterotrophic nitrification: possible factors controlling the post-disturbance mineral N flush in forest floors

A major forest disturbance such as clearcutting may bring on a flush of mineral N in organic forest floor horizons, but the magnitude of this flush can vary markedly from one ecosystem to another. For example, it was previously established that clearcutting in a high elevation Engelmann spruce-subalpine fir (ESSF) ecosystem results in significantly higher NH4+ and NO3− concentrations, whereas clearcutting in an old-growth coastal western hemlock (CWH) ecosystem has little effect on mineral N dynamics. We hypothesized that the higher mineral N flush observed in the ESSF ecosystem is due to a greater temperature sensitivity of mineral N transformation rates, and to a lower proportion of heterotrophic nitrifiers, compared to the CWH ecosystem. To test these two hypotheses, we sampled forest floors several times over the growing season from clearcut and old-growth plots in both ecosystems, and measured gross mineral N transformation rates at field temperatures and at 10 °C above field temperatures, as well as with and without acetylene to inhibit autotrophic nitrifiers. Gross NH4+ transformations rates ranged between 20 and 120 μg N (g forest floor)−1 day−1 at the ESSF site, and between 15 and 40 μg N (g forest floor)−1 day−1 at the CWH site. Higher temperature increased gross NH4+ transformation rates in forest floor samples at both sites, but the average Q10 value was higher at the ESSF site (3.15) than at the CWH site (1.25). Temperature sensitivity at the ESSF site was greater in clearcut plots (Q10=4.31) than in old-growth plots (Q10=1.98). Gross NO3− transformation rates ranged between 10 and 32 μg N (g forest floor)−1 day−1 at the ESSF site, and between 10 and 24 μg N (g forest floor)−1 day−1 at the CWH site, but there were no significant effects of temperature or clearcutting on gross NO3− transformation rates at either site. Likewise, there were no significant differences in the proportion of heterotrophic nitrifiers between sites. Overall, our results support the view that the temperature sensitivity of microbial processes may explain the magnitude of the NH4+ flush in some coniferous ecosystems, but we lack the evidence relating the magnitude of the NO3− flush to the proportion of heterotrophic nitrifiers.