Countercurrent electrophoresis on paper p

Assumptions concerning the similarity of paper chromatography and paper electrophoresis as regards the nature of the interaction of the substances analysed with the immobile phase were verified. glueckauf's method for constructing distribution isotherms from chromatographic measurements was applied to electrophoretic measurements. It was proved that the isotherms obtained in this way are identical with the isotherms obtained by the direct method, when the dependence of the mobility in free solution on the concentration can be neglected. The values of RF, the electrophoretic mobility in free solution Uo and the velocity uo obtained both by direct measurement and by calculation were proved to be identical.