Operation of the ISO-DALT system: Seventh edition

The ISO-DALT system of two-dimensional electrophoresis was developed as a series of modifications of the original technique of O'Farrell. Since the appearance of the first two open literature publications describing the ISO-DALT system in 1978, the technique has continually been refined. As a supplement to these first references, and to those that have followed, we have written a series of recipes and more detailed laboratory procedures that incorporate refinements and tricks of the trade as they have developed during our use of the system. The present collection is the 6th version in this constantly evolving series, and represents the state of art as of October, 1981. This, the sixth edition, has been prepared in order to bring up to date the changes that we have found helpful, as well as to add a few new details. (ERB)