Silica-based supports for high-performance liquid chromatography of biopolymers using non-denaturing conditions

Silica-based supports have been used successfully as media for high-performance liquid chromatography (HPLC) of proteins. Their characteristics of particle diameter, pore diameter, rigidity and suitability for derivatization have made them well accepted for all relevant HPLC techniques. In this article, physical properties and operating parameters of silica-based supports for those HPLC modes which render proteins intact and biologically active will be discussed.