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In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract
- Source :
- Revista Fitos. 15:136-143
- Publication Year :
- 2021
- Publisher :
- Fiocruz - Instituto de Tecnologia em Farmacos, 2021.
-
Abstract
- This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.
- Subjects :
- 0303 health sciences
food.ingredient
biology
Traditional medicine
010405 organic chemistry
Ethyl acetate
General Medicine
biology.organism_classification
Antimicrobial
01 natural sciences
0104 chemical sciences
Palmitic acid
Hexane
03 medical and health sciences
chemistry.chemical_compound
food
chemistry
Alpinia zerumbet
Agar
Gas chromatography
030304 developmental biology
Dichloromethane
Subjects
Details
- ISSN :
- 24464775 and 18089569
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Revista Fitos
- Accession number :
- edsair.doi...........739bd9646502fde6ae2a607ca69fd59d
- Full Text :
- https://doi.org/10.32712/2446-4775.2021.1037