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In vitro antimicrobial activity of Alpinia zerumbet and A. purpurata nonpolar fraction of leaf extract

Authors :
Ricardo Machado Kuster
Davi Oliveira e Silva
Daniela S. Alviano
Cristiane Pimentel Victório
Celuta S. Alviano
Celso Luiz Salgueiro Lage
Source :
Revista Fitos. 15:136-143
Publication Year :
2021
Publisher :
Fiocruz - Instituto de Tecnologia em Farmacos, 2021.

Abstract

This study aimed to evaluate the in vitro antimicrobial activity of fractions of leaf hydroalcoholic crude extracts: hexane, dichloromethane, ethyl acetate and butanolic of Alpinia zerumbet and A. purpurata by the agar drop diffusion method in order to screen the main compounds involved in antimicrobial activity. Leaves of A. zerumbet (Pers.) B.L. Burtt et R.M. Sm. and A. purpurata (Vieill) K. Schum adult plants were collected and then dried and macerated in 70% ethanol. Leaf extracts were further partitioned using solvents in increasing polarity. Dichloromethane fractions were analyzed by gas chromatography/mass spectrometry (GC/MS). The major compound in the dichloromethane fraction of both species was the hexadecanoic acid (palmitic acid). By drop diffusion assay, the antimicrobial activity of crude extract, as well as fractions of hexane, dichloromethane, ethyl acetate and butanolic, was evaluated against pathogenic bacteria and fungi. No bacteria were inhibited. However, the dichloromethane fraction exhibited promising antifungal activity against the following fungi tested: Cryptococcus neoformans, Fonsecaea pedrosoi, Trichophytoon rubrum, Microsporium canis and M. gypseum.

Details

ISSN :
24464775 and 18089569
Volume :
15
Database :
OpenAIRE
Journal :
Revista Fitos
Accession number :
edsair.doi...........739bd9646502fde6ae2a607ca69fd59d
Full Text :
https://doi.org/10.32712/2446-4775.2021.1037