Back to Search
Start Over
Restricted Rabbit Antibodies: Amino Acid Sequences of Rabbit H Chains of Allotype a1, a2, and a3 in the Region 80 to 94
- Source :
- The Journal of Immunology. 111:1838-1843
- Publication Year :
- 1973
- Publisher :
- The American Association of Immunologists, 1973.
-
Abstract
- The partial amino acid sequence of rabbit heavy (H) chains of allotype a1, a2, and a3 was determined. The H chains were derived from restricted antipolysaccharide antibodies which were shown to be precipitated quantitatively by antiallotype sera raised against pooled immunoglobulins. The N-terminal half of the H chains (C1 fragment) was isolated from a CNBr digest and subjected to automated Edman degradation. Extended homology with known sequences from rabbit pooled H chains indicated that C1 fragments were cleaved between residues 34 and 35 and/or 79 and 80. One additional cleavage site was observed at position 110 in two H chains. Section 80 to 94 of a1, a2, and a3 restricted H chains showed little sequence variation. No simple correlation between the identity of a single amino acid residue and the allotype of the H chain could be determined.
- Subjects :
- Immunology
Immunology and Allergy
Subjects
Details
- ISSN :
- 15506606 and 00221767
- Volume :
- 111
- Database :
- OpenAIRE
- Journal :
- The Journal of Immunology
- Accession number :
- edsair.doi...........72aad111fc29a8a6f2bbb5a7ae163b91