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Analysis of Gene Expression in Fasciola hepatica Juveniles and Adults by In Situ Hybridization
- Source :
- Methods in Molecular Biology ISBN: 9781071604748
- Publication Year :
- 2020
- Publisher :
- Springer US, 2020.
-
Abstract
- In situ hybridization (ISH) is a technique used for the spatial localization of nucleic acids within tissues and cells. It is based on the ability of labeled nucleic acids (probes) to hybridize under the right conditions with the nucleic acids present in fixed biological specimens. In this chapter, we describe protocols for detection of RNA by ISH using digoxigenin (DIG)-labeled probes for Fasciola hepatica adults (in cryosections, given their large size) and for newly excysted juveniles (NEJs, which are ideally suited given their small size for whole-mount ISH). We describe fluorogenic and chromogenic protocols, respectively, but the detection methods can be easily interchanged by using the appropriate enzyme-conjugated antibodies and detection solutions.
- Subjects :
- 0303 health sciences
Fasciola
biology
040301 veterinary sciences
RNA
04 agricultural and veterinary sciences
In situ hybridization
biology.organism_classification
Molecular biology
0403 veterinary science
03 medical and health sciences
chemistry.chemical_compound
chemistry
Dig
Gene expression
Nucleic acid
Digoxigenin
Fasciola hepatica
030304 developmental biology
Subjects
Details
- ISBN :
- 978-1-07-160474-8
- ISBNs :
- 9781071604748
- Database :
- OpenAIRE
- Journal :
- Methods in Molecular Biology ISBN: 9781071604748
- Accession number :
- edsair.doi...........71748346121957f9308be59f374a4fc6