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The role of antifreeze proteins in the regulation of genes involved in the response of Hosta capitata to cold
- Source :
- 3 Biotech. 9
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Cold temperatures are a major source of stress for plants and negatively impact crop yield. A possible way to protect plants is to treat them with antifreeze proteins (AFPs). Here, we investigated whether fish AFPs can shield the rare ornamental species Hosta capitata from low-temperature stress. We elucidated the expression patterns of the cold-inducible genes C-repeat binding factor 1 (CBF1) and dehydrin 1 (DHN1), as well as the antioxidant genes superoxide dismutase (SOD) and catalase (CAT). All were upregulated at low temperature (4 °C). With increasing exposure time, CBF1 and DHN1 expression generally rose (except CBF1 at 48 h). In contrast, SOD and CAT expression gradually declined from 6 to 48 h. Depending on exposure duration, AFP regulation of gene transcription varied with concentration. However, compared with other concentrations, 100 µg/L AFP reduced CBF1 and DHN1 expression and increased SOD and CAT expression in plants, regardless of exposure time. Both AFP I and III were likely to be most effective at protecting plants against cold stress at concentrations of 100 µg/L. Their involvement in H. capitata cold-stress treatment occurred through regulating the expression of important stress-response genes.
- Subjects :
- chemistry.chemical_classification
Reactive oxygen species
Antioxidant
Hosta capitata
biology
medicine.medical_treatment
Environmental Science (miscellaneous)
biology.organism_classification
Agricultural and Biological Sciences (miscellaneous)
Molecular biology
Superoxide dismutase
chemistry
Antifreeze protein
Catalase
Gene expression
biology.protein
medicine
Gene
Biotechnology
Subjects
Details
- ISSN :
- 21905738 and 2190572X
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- 3 Biotech
- Accession number :
- edsair.doi...........70df1075e3bca38b35746537c4db70cc