Back to Search
Start Over
Titania nanotube-based protein delivery system to inhibit cranial bone regeneration in Crouzon model of craniosynostosis
- Source :
- International Journal of Nanomedicine. 14:6313-6324
- Publication Year :
- 2019
- Publisher :
- Informa UK Limited, 2019.
-
Abstract
- Background: Craniosynostosis is a developmental disorder characterized by the premature fusion of skull sutures, necessitating repetitive, high-risk neurosurgical interventions throughout infancy. This study used protein-releasing Titania nanotubular implant (TNT/Ti) loaded with glypican 3 (GPC3) in the cranial critical-sized defects (CSDs) in Crouzon murine model (Fgfr2c342y/+ knock-in mutation) to address a key challenge of delaying post-operative bone regeneration in craniosynostosis. Materials and methods: A 3 mm wide circular CSD was created in two murine models of Crouzon syndrome: (i) surgical control (CSDs without TNT/Ti or any protein, n=6) and (ii) experimental groups with TNT/Ti loaded with GPC3, further subdivided into the presence or absence of chitosan coating (on nanotubes) (n=12 in each group). The bone volume percentage in CSDs was assessed 90 days post-implantation using micro-computed tomography (micro-CT) and histological analysis. Results: Nano-implants retrieved after 90 days post-operatively depicted well-adhered, hexagonally arranged, and densely packed nanotubes with average diameter of 120±10 nm. The nanotubular architecture was generally well-preserved. Compared with the control bone volume percentage data (without GPC3), GPC3-loaded TNT/Ti without chitosan coating displayed a significantly lower volume percent in cranial CSDs (P
- Subjects :
- X-ray microtomography
Biophysics
Pharmaceutical Science
Bioengineering
02 engineering and technology
010402 general chemistry
01 natural sciences
Craniosynostosis
Biomaterials
In vivo
Drug Discovery
medicine
Bone regeneration
Chemistry
Regeneration (biology)
Organic Chemistry
Crouzon syndrome
General Medicine
021001 nanoscience & nanotechnology
medicine.disease
0104 chemical sciences
Skull
medicine.anatomical_structure
Implant
0210 nano-technology
Biomedical engineering
Subjects
Details
- ISSN :
- 11782013
- Volume :
- 14
- Database :
- OpenAIRE
- Journal :
- International Journal of Nanomedicine
- Accession number :
- edsair.doi...........708720159ed418d975e5d6dd76d4dd5f