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NITRIC OXIDE INHIBITORY ACTIVITY OF STRYCHNOS SPINOSA (LOGANIACEAE) LEAF EXTRACTS AND FRACTIONS
- Source :
- African Journal of Traditional, Complementary and Alternative Medicines. 13:22-26
- Publication Year :
- 2016
- Publisher :
- African Traditional Herbal Medicine Supporters Initiative (ATHMSI), 2016.
-
Abstract
- Background: The study was aimed at determining the anti-inflammatory activity of fractions and extracts obtained from Strychnos spinosa leaves on a mediator of inflammation nitric oxide (NO). Materials and Methods: Leaves were extracted with acetone and separated into fractions with different polarities by solventsolvent fractionation. The Griess assay was used to determine the nitric oxide (NO) inhibitory activity. Cellular toxicity was determined by "using the MTT reduction assay". Results: With the exception of the ethyl acetate fraction which had an IC50 >750 μg/mL, all extracts and fractions had significant nitric oxide-inhibitory activity. The most active being the water fraction, chloroform fraction and the dichloromethane/methanol extracts with IC50 values of 88.43 μg/mL, 96.72 μg/mL and 115.62 μg/mL, respectively. The extracts and fractions had low cytotoxicity on macrophage U937 cell lines. Conclusion: Extracts and fractions of Strychnos spinosa leaves may be promising sources of natural anti-inflammatory agents. Findings obtained from this study showed that Strychnos spinosa leaves possess promising anti-inflammatory action and could be used in the treatment of inflammation-related conditions.
- Subjects :
- 0301 basic medicine
Chloroform
Chromatography
biology
Ethyl acetate
Loganiaceae
Fractionation
biology.organism_classification
01 natural sciences
0104 chemical sciences
Nitric oxide
010404 medicinal & biomolecular chemistry
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Complementary and alternative medicine
chemistry
Biochemistry
Strychnos spinosa
Drug Discovery
Acetone
Dichloromethane
Subjects
Details
- ISSN :
- 25050044 and 01896016
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- African Journal of Traditional, Complementary and Alternative Medicines
- Accession number :
- edsair.doi...........707dd5abc6e8cd105dbe5be0d46d3ba0