Oxidations in the tricarboxylic acid cycle by intact mitochondria isolated from the lateral red muscle of goldfish (Carassius auratus L.). Effects of anoxia on the oxidation of pyruvate and glutamate

1. 1. Oxygen uptake and 14 CO 2 production by goldfish red muscle mitochondria in the presence of various substrates were examined. 2. 2. Isolated mitochondria demonstrate a high efficiency of oxidative phosphorylation and respiratory control ratios ranging from 3.6 to 14.6. 3. 3. In the presence of citrate, isocitrate, fumarate or NADH the respiratory rate is not stimulated by added ADP. 4. 4. Pyruvate is oxidized without addition of a sparker metabolite. 5. 5. No activity of the glycerol phosphate shuttle could be induced. 6. 6. Anoxia and ADP stimulate 14 CO 2 production from [1- 14 C] pyruvate, [2- 14 C] pyruvate and [U- 14 C] glutamate. 7. 7. It is suggested that the regulation of the pyruvate dehydrogenase complex activity is modified during anoxia.