Quantitative expression of ING2-mRNA in invasive cancer of the colon and rectum

Colorectal cancer is the third and second, respectively, most common form of cancer in the male and female populations, according to new cases reported at the global level. The aim of this study was to contribute to the understanding of the role of the inhibitor of growth gene - 2 (ING2-mRNA) protein in colorectal cancer, raising its potential to the prognostic level. The research sample consisted of 60 patients (60% men, mean age 73.2 ± 11.1 years) who underwent surgery for colorectal cancer during the period 2008-2013 in the Asklipieio Voulas General Hospital. Samples from tumor tissue mucosal were compared with normal ones and the levels of ING2-mRNA were defined quantitively through Real Time Polymerase Chain Reaction (RT-PCR). Finally, these levels were correlated with the correspond pathological image and characteristics of each patient, using the SPPS V.20 program for statistical process. After Total RNA was extracted, the primers used were listed as follows: ING2 (sense: 5’-GCAGGCAGCGGACCAGTGAA-3’, antisense: 5’-CCTGCTTGGCCTTGGAGCGT-3’), GAPDH (sense: 5’-GAAATCCCATCACCATCTTCCAGG-3’, antisense: 5’-GAGCCCCAGCCTTCTCCATG-3’). The results of the study confirmed the marginal correlation already documented between negative expression of ING2-mRNA and stage 2a of the TNM staging of colorectal cancer. These results lead to the conclusion that the level of this specific mRNA can be prognostic of clinical outcome. Further research is needed, both to corroborate this correlation and to elucidate the mechanism of ING2 action in colorectal cancer.