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Conformation and Physical Structure of Tropoelastin from Human Vascular Cells: Influence of Cells Lipid Loading

Authors :
Jany Dandurand
Lina Badimon
Laura Nasarre
Vicenta Llorente-Cortés
Valérie Samouillan
Colette Lacabanne
Source :
Conference Papers in Science. 2014:1-4
Publication Year :
2014
Publisher :
Hindawi Limited, 2014.

Abstract

Aggregated low density lipoproteins (agLDL) contribute to massive intracellular cholesteryl ester (CE) accumulation in human vascular smooth muscle cells (VSMC). Our aim was to determine the conformational and physical structure of agLDL and elastic material produced either by control human VSMC or by agLDL-loaded human VSMC (agLDL-VSMC). At the conformational level scanned by FTIR spectroscopy, a new undefined, probably non-H-bonded, structure for tropoelastin produced by agLDL-VSMC is revealed. By differential scanning calorimetry, a decrease of water affinity and a drop of the glass transition associated with aggregated tropoelastin (from 200°C to 159°C) in the supernatant from agLDL VSMC are evidenced. This second phenomenon is due to an interaction between agLDL and tropoelastin as detected by the weak specific FTIR absorption band of agLDL in supernatant from agLDL-loaded VSMC.

Details

ISSN :
23566094 and 23566108
Volume :
2014
Database :
OpenAIRE
Journal :
Conference Papers in Science
Accession number :
edsair.doi...........6d97aa958869e398b6f1ea8fa0d6215d
Full Text :
https://doi.org/10.1155/2014/391242