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In vivo targeting of breast cancer with peptide functionalized GQDs/hMSN nanoplatform
- Source :
- Journal of Nanoparticle Research. 21
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Graphene quantum dots capped in hollow mesoporous silica nanoparticles (GQDs@hMSN) exhibited great potential in medical applications due to its good optical properties and high drug loading capacity. Compared with antibodies, peptide has a better affinity with target proteins. Herein, we demonstrated efficient targeting of triple-negative breast cancer with GQDs@hMSN, which was conjugated to a peptide ligand, F3 against nucleolin, to form GQDs@hMSN-F3. The core/shell GQDs@hMSN and GQDs@hMSN-F3 had diameters of 100 nm and 130 nm, respectively, based on transmission electron microscope (TEM) and dynamic laser scattering (DLS) measurement. Doxorubicin (DOX) was loaded onto GQDs@hMSN with a relatively high loading capacity. Systematic in vitro and in vivo studies were performed to investigate the targeting specificity and tissue distribution of GQDs@hMSN conjugates. Fluorescence microscopy examination and flow cytometry confirmed the targeting specificity of F3-attached GQDs@hMSN conjugates against cell nucleolin. A more potent uptake of GQDs@hMSN-F3 in MDA-MB-231 nodules was witnessed when compared with that of non-targeted GQDs@hMSN. Based on the findings from cellular targeting and in vivo fluorescence imaging, F3-attached GQDs@hMSN conjugates had the potential to serve as an image-guidable, tumor-selective cargo delivery nanoplatform.
- Subjects :
- Materials science
Bioengineering
Peptide
02 engineering and technology
010402 general chemistry
01 natural sciences
Flow cytometry
In vivo
Fluorescence microscope
medicine
General Materials Science
chemistry.chemical_classification
medicine.diagnostic_test
General Chemistry
Mesoporous silica
021001 nanoscience & nanotechnology
Condensed Matter Physics
Atomic and Molecular Physics, and Optics
0104 chemical sciences
chemistry
Modeling and Simulation
Drug delivery
Biophysics
Nanomedicine
0210 nano-technology
Nucleolin
Subjects
Details
- ISSN :
- 1572896X and 13880764
- Volume :
- 21
- Database :
- OpenAIRE
- Journal :
- Journal of Nanoparticle Research
- Accession number :
- edsair.doi...........6d703acc2474ff20f1db51575de5c31b