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Adoption of pelleted Digitalia iburua grain as carrier for heat stable Newcastle disease vaccine virus for village poultry

Authors :
P. Abdu
M. Usman
John Osita Arinze Okoye
G. O. N. Echeonwu
J. S. Rabo
O. J. Ibu
A. I. Shittu
A. Egbuji
A. Lohlum
D. C. Eze
E. C. Okwor
Source :
African Journal of Biotechnology. 16:2050-2053
Publication Year :
2017
Publisher :
Academic Journals, 2017.

Abstract

The development of pelleted food–based vaccine against Newcastle disease (ND) using a thermostable NDVI2 strain in Acha (Digitalia iburua) is reported. The Acha cereal was subjected to rigorous processing of washing, soaking, boiling, roasting and grinding into fine powder prior to virus incorporation. The mixture which was passed through a locally fabricated pelleting machine resulted in fine pellets. The virus content in the pellets determined by inoculation of susceptible embryonated chicken eggs showed appreciable titres above 108 Egg Infective Dose 50% end points (EID50) per g of feed. These titres appear to be higher than the recommended minimum immunizing dose of 105.5 EID50 per ml of vaccine. Further in-vitro and in-vivo assessment of the pelleted vaccine is advocated. Key words: Pelleted, NDVI2, food vaccine, Digitalia iburua, high virus titres.

Details

ISSN :
16845315
Volume :
16
Database :
OpenAIRE
Journal :
African Journal of Biotechnology
Accession number :
edsair.doi...........6c4a0398dceda5fa913e1f290e18f8c0
Full Text :
https://doi.org/10.5897/ajb2017.15996