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Prolonged Immersion Improves the Effectiveness of Dilute Vibrio Vaccine for Rainbow Trout

Authors :
Teruyuki Nakanishi
Mitsuru Ototake
James D. Moore
Source :
Fish Pathology. 34:151-154
Publication Year :
1999
Publisher :
The Japanese Society of Fish Pathology, 1999.

Abstract

In order to clarify the effect of a prolonged exposure period on vaccine effectiveness, juvenile rainbow trout were immersed in 100, 1, 000 and 10, 000 times dilutions of a commercial vibrio bacterin for 3 min (brief immersion, BI) and for 24 h (prolonged immersion, PI). Two weeks and 2, 4 and 6 mo after the vaccination, fish were challenged by waterborne exposure to Vibrio ordalii. Prolonged exposure to dilute bacterin resulted in better protection than the standard BI method. Two weeks after vaccination, the cumulative mortality was 0% in the group vaccinated by PI with the 100, 1, 000 and 10, 000 times diluted vaccine. In contrast, mortality was 58 and 77% in the groups vaccinated by BI with the 100 and 10, 000 times diluted vaccine respectively, and was 100% in the unimmunized control group. Furthermore, 6 mo after vaccination, the mortality was 20% (100 times dilution) to 77% (10, 000 times dilution) in the PI group, while it was 64% (100 times dilution) to 93% (10, 000 times dilution) in the BI group. Mortality in the corresponding control group was 97%. These results suggest that prolonged exposure period improves vaccine effectiveness.

Details

ISSN :
18817335 and 0388788X
Volume :
34
Database :
OpenAIRE
Journal :
Fish Pathology
Accession number :
edsair.doi...........6c0ce673ee6e3f23f37963b2df37b186
Full Text :
https://doi.org/10.3147/jsfp.34.151