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Efficient Biosynthesis of Raspberry Ketone by Engineered Escherichia coli Coexpressing Zingerone Synthase and Glucose Dehydrogenase
- Source :
- Journal of Agricultural and Food Chemistry. 69:2549-2556
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- Raspberry ketone (RK), the main aroma compound of raspberry fruit, has applications in cosmetics, food industry, and pharmaceutics. In this study, we biosynthesized RK via the catalytic reduction of 4-hydroxybenzylidenacetone using a whole-cell biocatalyst. Reductase RiRZS1 from Rubus idaeus and glucose dehydrogenase SyGDH from Thermoplasma acidophilum were expressed in Escherichia coli to regenerate NADPH for the whole-cell catalytic reaction. Following the optimization of balancing the coexpression of two enzymes in pRSFDuet-1, we obtained 9.89 g/L RK with a conversion rate of 98% and a space-time yield of 4.94 g/(L·h). The optimum conditions are 40 °C, pH 5.5, and a molar ratio of substrate to auxiliary substrate of 1:2.5. Our study findings provide a promising method of biosynthesizing RK.
- Subjects :
- 0106 biological sciences
Zingerone
chemistry.chemical_classification
biology
010401 analytical chemistry
Raspberry ketone
Thermoplasma acidophilum
General Chemistry
Reductase
biology.organism_classification
medicine.disease_cause
01 natural sciences
0104 chemical sciences
Blowing a raspberry
chemistry.chemical_compound
Enzyme
chemistry
Biochemistry
Glucose dehydrogenase
medicine
General Agricultural and Biological Sciences
Escherichia coli
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi...........6bf2b1a48bbf515ddfe9f2650cb7b071