Additional file 3: Figure S1. of MitoRS, a method for high throughput, sensitive, and accurate detection of mitochondrial DNA heteroplasmy

Overview of the MitoRS wet lab method. Total DNA (5 ng) is amplified by RCA. Sequencing libraries are subsequently generated using the Nextera XT kit from Illumina, starting from 1 ng amplification product. Libraries are pooled at equimolar ratios and sequenced on a HiSeq 2500 (Illumina) using rapid mode for a paired-end run of 2 x 150 cycles. (PPTX 40 kb)