A ratiometric fluorescent and colorimetric probe for selective detection of hydrazine

A colorimetric and ratiometric fluorescent hydrazine reactive probe COUMA2 has been developed. In aqueous solution, hydrazinolysis of COUMA2 results in deacetylation affording fluorescent products displaying dual emissive peaks at 500 and 655 nm. 50 μM NH2NH2 can induce a ratiometric change of fluorescent signal from COUMA2 by a factor of 4.89.