486. Optimized Conditions for Retroviral Gene Transfer by RetroNectin® Bound Virus Infection Method

In retroviral gene transduction, the efficiency of viral infection is suppressed by the proteoglycans and some other materials secreted by the producer cell lines. In order to remove these inhibitors, we previously developed the rFN-CH-296 (RetroNectin®)-bound virus infection method. In the present study, we further optimized the conditions of gene transfer efficiency into human leukemia cell lines, K562, TF-1, HL-60, and CEM cells utilizing RetroNectin® plates with galv-pseudo-typed or amphotropic retrovirus vector, and applied the optimized protocol to the CD34+ BMCs in a closed system with gas permeable culture bag.