Additional file 8: Figure S7. of A positive feedback loop involving the Wnt/β-catenin/MYC/Sox2 axis defines a highly tumorigenic cell subpopulation in ALK-positive anaplastic large cell lymphoma

Overexpression of MYC significantly attenuates the decreased SRR2 luciferase activity induced by inhibition of β-catenin by using quercetin. The SRR2 luciferase activity in RR cells derived from Karpas 299 with EV or MYC transfection in the presence of 50 μM quercetin for 24 h; cells with DMSO treatment were included as a negative control. The SRR2 luciferase activity decreased by ~50% in RR cells from Karpas 299 with EV transfection upon quercetin treatment, whereas it only decreased by ~25% in cells with MYC transfection. The MYC transfection efficiency was validated in Figure S2c. (PDF 36 kb)