SIMULTANEOUS QUANTIFICATION OF NINE BIOACTIVE CONSTITUENTS IN DENGZHANXIXIN INJECTION BY UPLC-MS/MS

In this study, a rapid and comprehensive ultra-performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) method was developed for simultaneous determination of nine major bioactive constituents in Dengzhanxixin injection. The chromatographic separations were obtained on an Acquity UPLC BEH C18 column (50 mm × 2.1 mm I.D., 1.7 μm) by a linear gradient elution with the mobile phase consisting of A (water and 0.6% acetic acid) and B (acetonitrile and 0.6% acetic acid). The total analytical run time was relatively short (14 min). The analytes and two internal standards (ISs) were all monitored in a selected-ion reaction (SIR) mode with a negative electrospray ionization (ESI−) interface. The calibration curves of all analytes revealed good linear regression (r2 ≥ 0.9972) within test ranges. This method provided good precision with RSD of intra- and interday variation less than 1.26% and 2.51%, respectively. The validated method was then applied to quantify the nine major constituents in six batc...