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Thermal and vibrational characterization of human skin
- Source :
- Journal of Thermal Analysis and Calorimetry. 127:1143-1154
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- For a better understanding of the molecular and organizational changes in human dermis, biophysical methods were tested. The aim of this study was to find suitable and reproducible biomarkers for further clinical studies on intrinsic and extrinsic aging of dermis. Thermoporometry, hydric organization and thermal transitions of fresh and frozen skins were determined by differential scanning calorimetry (DSC). Fourier transform infrared spectroscopy (FTIR) was used to identify the absorption bands of the dermis especially in the 1800–1000 cm−1 zone and to discriminate between the different secondary structures of proteins. A widening of the pore size distribution is evidenced with freezing, but there is no significant difference between the hydric organization and the endothermic collagen denaturation of fresh and frozen skins. The global FTIR spectra and the second derivative spectra in the scanned zone are also identical in fresh and frozen dermis, validating the storage protocol. DSC and FTIR are well-suited techniques to characterize human skin, giving accurate results with high reproducibility. The acquisition of thermal and vibrational biomarkers of the skin at the mesoscale and nanoscale contributes to its better knowledge and is promising for further studies on skin aging.
- Subjects :
- Materials science
integumentary system
010401 analytical chemistry
Human skin
02 engineering and technology
Absorption (skin)
021001 nanoscience & nanotechnology
Condensed Matter Physics
01 natural sciences
Endothermic process
0104 chemical sciences
Characterization (materials science)
Skin Aging
Differential scanning calorimetry
Nuclear magnetic resonance
medicine.anatomical_structure
Dermis
medicine
Biophysics
Physical and Theoretical Chemistry
Fourier transform infrared spectroscopy
0210 nano-technology
Subjects
Details
- ISSN :
- 15882926 and 13886150
- Volume :
- 127
- Database :
- OpenAIRE
- Journal :
- Journal of Thermal Analysis and Calorimetry
- Accession number :
- edsair.doi...........6abeb44829c9e9073bfbefbd001bdb7c
- Full Text :
- https://doi.org/10.1007/s10973-016-5384-z