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A comparative genomic hybridization approach to study gene copy number variations among chinese hamster cell lines
- Source :
- Biotechnology and Bioengineering. 114:1903-1908
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Chinese Hamster Ovary (CHO) cells are aneuploid in nature. The genome of recombinant protein producing CHO cell lines continuously undergoes changes in its structure and organization. We analyzed nine cell lines, including parental cell lines, using a comparative genomic hybridization (CGH) array focused on gene-containing regions. The comparison of CGH with copy-number estimates from sequencing data showed good correlation. Hierarchical clustering of the gene copy number variation data from CGH data revealed the lineage relationships between the cell lines. On analyzing the clones of a clonal population, some regions with altered genomic copy number status were identified indicating genomic changes during passaging. A CGH array is thus an effective tool in quantifying genomic alterations in industrial cell lines and can provide insights into the changes in the genomic structure during cell line derivation and long term culture. Biotechnol. Bioeng. 2017;114: 1903-1908. © 2017 Wiley Periodicals, Inc.
- Subjects :
- 0301 basic medicine
Genetics
Lineage (genetic)
biology
Chinese hamster ovary cell
Bioengineering
biology.organism_classification
Applied Microbiology and Biotechnology
Genome
Chinese hamster
law.invention
03 medical and health sciences
030104 developmental biology
law
Cell culture
Recombinant DNA
Copy-number variation
Biotechnology
Comparative genomic hybridization
Subjects
Details
- ISSN :
- 00063592
- Volume :
- 114
- Database :
- OpenAIRE
- Journal :
- Biotechnology and Bioengineering
- Accession number :
- edsair.doi...........68f2d7e36370759f92446fbd64fb4be2