High yield chromatographic purification of enzymatically produced tyrosine fructosyl ester

The synthesis of L-tyrosine fructosyl ester, from fructose and L-tyrosine methyl ester, was carried out by a transesterification reaction catalyzed by α-chymotrypsin in water without any organic cosolvent. The yield was optimized by regulating the pH of the reaction medium and a maximum yield of 63% was obtained.