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Fusion of hamster and pig zona-free eggs stimulated by boar and guinea pig sperm at fertilization in vitro
- Source :
- Gamete Research. 6:189-197
- Publication Year :
- 1982
- Publisher :
- Wiley, 1982.
-
Abstract
- In the course of in vitro fertilization of zona-free hamster and pig eggs by boar and guinea-pig spermatozoa it was observed that homologous and heterologous eggs fused together, forming cell hybrids between two or more cells. The fusogenic activity was attributed to spermatozoa and this was the hypothesis tested. The fusogenic activity (coinciding with sperm penetration activity) was dependent on the duration of sperm preincubation, which may be regarded as capacitation in vitro. Fusion occurred only after 3 hr of sperm preincubation and a narrow optimum was detected at 4–4.5 hr. Fusion of eggs was also dependent on sperm concentration. A relatively high proportion of fusions was observed at a sperm concentration of 4.0 × 104 per ml and an optimum was attained at a concentration of 5.0 × 105 per ml. The first fusions were observed at 90 min after semination. After 3 hr more than a half of the eggs reacted, and by 20 hr of incubation 80% of ova were fused. The fusability of eggs was tested and found to occur at 14 hr after ovulation. The fusion process was also studied using transmission electron microscopy. It is supposed that the process of egg fusion may be caused either by a similar mechanism to sperm-egg fusion, or by products released during the sperm acrosome reaction.
- Subjects :
- endocrine system
In vitro fertilisation
BOAR
urogenital system
media_common.quotation_subject
medicine.medical_treatment
Acrosome reaction
Hamster
Biology
Sperm
Andrology
Human fertilization
Capacitation
Botany
Genetics
medicine
Ovulation
reproductive and urinary physiology
Developmental Biology
media_common
Subjects
Details
- ISSN :
- 01487280
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Gamete Research
- Accession number :
- edsair.doi...........685de9023de3c7c0cf71eb19b6e5bd1d
- Full Text :
- https://doi.org/10.1002/mrd.1120060302