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A MONOCLONAL ANTIBODY TO HUMAN PLACENTAL LACTOGEN HORMONE FACILITATES ISOLATION OF FETAL CELLS FROM MATERNAL BLOOD IN A MODEL SYSTEM
- Source :
- Prenatal Diagnosis. 16:813-821
- Publication Year :
- 1996
- Publisher :
- Wiley, 1996.
-
Abstract
- A cocktail of trophoblast-reactive monoclonal antibodies (MAbs) is required for efficient isolation of trophoblasts from maternal blood. A modified antibody screening procedure was used to identify a clone, in a COS cell placental cDNA expression library, that expressed the gene product recognized by MAb FDO202N. The antigen recognised by MAb FDO202N was identified as human placental lactogen (hPL) hormone. hPL hormone is secreted into the maternal blood by trophoblasts at high levels during pregnancy. Immunohistochemical localization of hPL hormone was consistent with expression in the syncytiotrophoblast and extravillous cytotrophoblast. A model system was used where known numbers of syncytiotrophoblast sprouts were seeded into saline or maternal blood, bound by trophoblast-specific MAbs, recovered magnetically, and then counted. MAb FDO202N was shown to facilitate the efficient recovery of trophoblast sprouts from saline and maternal blood.
- Subjects :
- medicine.medical_specialty
medicine.drug_class
Obstetrics and Gynecology
Trophoblast
Biology
Monoclonal antibody
female genital diseases and pregnancy complications
Andrology
Syncytiotrophoblast
medicine.anatomical_structure
Endocrinology
Human placental lactogen
Antigen
Internal medicine
embryonic structures
medicine
Immunohistochemistry
Placental lactogen
Clone (B-cell biology)
reproductive and urinary physiology
Genetics (clinical)
Subjects
Details
- ISSN :
- 10970223 and 01973851
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Prenatal Diagnosis
- Accession number :
- edsair.doi...........682663c494dbda8c848264ed2e4cfc6d