Leptospira culture and isolation from tertiary care hospital, Surat

Background: In a highly globalized world, where boundaries are continually crossed due to different international activities, tropical diseases including Leptospirosis have emerged as significant causes of severe illness and death in untreated patients. Aim: To optimize the culture isolation of Leptospira by using different culture methods. Materials and Methods: All suspected cases of Leptospirosis regardless of their age/sex were recruited following informed and written consent. Five different blood culture methods have been tried to see better isolation of Leptospira from patient. Result: Total 326 patients are evaluated over a period of three years. Five different method of blood culture were followed out of that, whole blood culture technique shows better result compaire to other followed technique. Conclusion: The culture methods are gold standard but time consuming. It has an important role in the study of outbreaks. It provides epidemiological data that is helpful in studying pathogenesis of Leptospira. Keywords: Leptospirosis, Blood culture, Isolation, EMJH medium.