598 Stimulation of Human neutrophils functions in vitro and after oral administration of a polyenzyme preparation

Polymorphonuclear neutrophils (PMN) can be primed for enhanced release of reactive oxygen species (ROS) by exposure to cytokines and biological response modifiers. ROS are considered to possess tumorici-dal activity. The polyenzyme preparation Wobenzym R (WE) contains pancreatin, papain, bromelain trypsin and chymotrypsin and is used in adjuvant tumor therapy. We investigated killing of WE exposed PMN against tumor cells and analyzed WE influence on ROS production in a chemiluminescence assay in PMN in vitro and in vivo . Depending on dose WE stimulates the cytotoxic capacity of PMN in vitro against tumor cells (50 μg/ml: P P P