Back to Search
Start Over
The Burholderia pyrrocinia Purple Acid Phosphatase Pap9 Mediates Phosphate Acquisition in Plants
- Source :
- Journal of Plant Biology. 62:342-350
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Increasing the expression level of purple acid phosphatases (PAPs), which hydrolyze organic phosphate to inorganic phosphate (Pi), is an important response to Pi starvation in plants. PAPs are widely distributed in eukaryotes like mammals and plants but distributed in limited microorganisms. A PAP-like protein named Pap9 from Burkholderia pyrrocinia CH-67 was isolated. The enzyme had optimal activity at atypical pH of 8.5 and temperature of 85°C. When pap9 was overexpressed in Arabidopsis thaliana, the APase activity in the transgenic plants was about 15% to 40% higher under acidic conditions and had an approximately four-fold increase under alkaline conditions compared with wild type. Overexpression of pap9 in plants enhanced APase activity under Pi-deficient conditions more than under Pi-sufficient conditions. In-gel assays revealed that Pap9 exists as a monomer in transgenic plants. Root surface-associated APase activity in transgenic plants increased dramatically during acidic and Pi-deficient conditions. These results indicated that bacterial Pap9 in transgenic plants was secreted onto the root surface and released into the rhizosphere. The transgenic plants have significantly enhanced biomass that have the potential for use in biotechnological applications.
- Subjects :
- 0106 biological sciences
0301 basic medicine
chemistry.chemical_classification
Rhizosphere
biology
fungi
Wild type
food and beverages
Plant Science
Genetically modified crops
Purple acid phosphatases
Phosphate
biology.organism_classification
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Enzyme
chemistry
Biochemistry
Burkholderia pyrrocinia
Arabidopsis thaliana
010606 plant biology & botany
Subjects
Details
- ISSN :
- 18670725 and 12269239
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Journal of Plant Biology
- Accession number :
- edsair.doi...........664cc752f55dd2d79e82f889b55abca4