Back to Search
Start Over
Immunomagnetic separation combined with RT-qPCR for evaluating the effect of disinfectant treatments against norovirus on food contact surfaces
- Source :
- LWT. 97:83-86
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Despite the importance of preventing the spread of human norovirus (NoV), effective disinfectants are lacking. In this study, the efficacy of disinfectants against NoV on various non-porous surfaces was investigated by immunomagnetic separation (IMS) and quantitative real-time reverse transcription (RT-q)PCR. Monoclonal anti-NoV antibody-conjugated magnetic beads captured intact viral particles for RT-qPCR after the treatment of surfaces with disinfectants. Viral suspensions of approximately 5.97 log10 genomic copies were inoculated onto rubber, glass, stainless steel, ceramic tile, wood, and polyvinyl chloride surfaces. Various elution buffers were used to elute NoV from surfaces. Ethanol (50–70%), sodium hypochlorite (NaOCl, 100–1000 ppm), sodium metasilicate (SMS, 0.1% and 0.3%), and quaternary compounds (1000–2000 ppm) were investigated as disinfectants; the observed reductions were
- Subjects :
- 0301 basic medicine
Chromatography
Food contact
Elution
Disinfectant
030106 microbiology
technology, industry, and agriculture
medicine.disease_cause
Immunomagnetic separation
03 medical and health sciences
Polyvinyl chloride
chemistry.chemical_compound
030104 developmental biology
chemistry
Sodium hypochlorite
Norovirus
medicine
Sodium metasilicate
Food Science
Subjects
Details
- ISSN :
- 00236438
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- LWT
- Accession number :
- edsair.doi...........66182563c2cc005d2e84c185e773d4ab