Additional file 3: Figure S3. of Retinoic acid-induced CHD5 upregulation and neuronal differentiation of neuroblastoma

NB69 morphology in response to 13cRA. (A) (A) Representative phase contrast images showing NB69 morphology change with or without 13cRA treatment (10 μM, 12 days). Two very different morphologic populations of cells were observed: a neuronal morphology with neurite outgrowth (N-type) (Red arrows), and flat and substrate adherent (S-type) (Blue arrows). (B) Immunofluorescence of NB69 cells with or without 13cRA treatment. Representative immunofluorescence images obtained when cells were treated with 13cRA and stained with neuronal markers TH, SYN, and βIII-Tubulin. (DOC 395 kb)