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Effects of abiotic stresses on the expression of chitinase-like genes in Acyrthosiphon pisum

Authors :
Chunchun Li
Inzamam Ul Haq
Aroosa Khurshid
Yan Tao
Peter Quandahor
Jing-Jiang Zhou
Chang-Zhong Liu
Source :
Frontiers in Physiology. 13
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

Insect chitinases play a crucial part to digest chitin in the exoskeleton during the molting process. However, research on insect chitinase related to the environmental stress response is very limited. This study was the first conducted to expression analysis of chitinase- related genes in A. pisum under abiotic stresses. Here, we identified five chitinase-like proteins (ApIDGF, ApCht3, ApCht7, ApCht10 and ApENGase), and clustered them into five groups (group II, III, V, Ⅹ, and ENGase). Developmental expression analysis revealed that the five A. pisum chitinase-related genes were expressed at whole developmental stages with different relative expression patterns. When aphids were exposed to various abiotic stresses including temperature, insecticide and the stress 20-hydroxyecdysone (20E), all five chitinase genes were differentially expressed in A. pisum. The results showed that insecticide such as imidacloprid down-regulated the expression of these five Cht-related genes. Analysis of temperature stress of A. pisum chitinase suggested that ApCht7 expression was high at 10°C, which demonstrates its important role in pea aphids under low temperature. Conversely, ApCht10 was more active under high temperature stress, as it was significantly up-regulated at 30°C. Besides, 20E enhanced ApCht3 and ApCht10 expression in A. pisum, but reduced ApCht7 expression. These findings provide basic information and insights for the study of the role of these genes under abiotic stress, which advances our knowledge in the management of pea aphids under multiple stresses.

Subjects

Subjects :
Physiology
Physiology (medical)

Details

ISSN :
1664042X
Volume :
13
Database :
OpenAIRE
Journal :
Frontiers in Physiology
Accession number :
edsair.doi...........65f130204637f1888ff334c2e4968770
Full Text :
https://doi.org/10.3389/fphys.2022.1024136