Multiple factors driving the acquisition efficiency of apple proliferation phytoplasma in Cacopsylla melanoneura

Phytoplasmas are bacterial pathogens located in the plant’s phloem that are responsible for several plant diseases and are mainly transmitted by phloem-sucking insects. Apple proliferation (AP) is an economically important disease caused by ‘CandidatusPhytoplasma mali’ which is transmitted by two psyllid species. While Cacopsylla picta is a confirmed vector in different regions, the vector efficiency of Cacopsylla melanoneura varies between different populations. This species is considered the main AP vector in Northwestern Italy but plays a minor role in Northeastern Italy and other European regions. To investigate whether the psyllid and/or the phytoplasma subtype drive the phytoplasma acquisition in C. melanoneura, we set-up a phytoplasma acquisition experiment using single mating couples including different psyllid populations and phytoplasma subtypes. All analyzed insect populations acquired phytoplasma, but with different efficiencies and concentrations. The main factors driving the acquisition were the phytoplasma subtype and its concentration in the leaves of the infected trees together with the psyllid family. The phytoplasma concentration in the psyllids was again influenced by the phytoplasma subtype, the psyllid family and the region of origin, whereas the phytoplasma concentration in the leaves and the psyllid haplotype defined with the cytochrome oxidase I gene had only a minor impact on the phytoplasma concentration. This is the first study evaluating the roles of both the psyllid haplotype and the phytoplasma subtype on the acquisition process shedding new light on the importance of C. melanoneura as AP vector.