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New ribosome-inactivating proteins from seeds and fruits of the bitter gourd Momordica charantia
- Source :
- The International Journal of Biochemistry & Cell Biology. 31:895-901
- Publication Year :
- 1999
- Publisher :
- Elsevier BV, 1999.
-
Abstract
- A new ribosome-inactivating protein (RIP) (δ-momorcharin) and a candidate RIP (e-momorcharin) were isolated, respectively, from the seeds and fruits of the bitter gourd Momordica charantia, by affinity chromatography on Affi-gel blue gel and ion exchange chromatography on Mono S with a fast protein liquid chromatography (FPLC) system. Both δ- and e-momorcharins were adsorbed on Affi-gel blue gel and were eluted from the cation exchange Mono S column earlier than α- and β-momorcharins, the two previously reported RIPs, δ- and e-momorcharins possessed a molecular weight of 30 and 24 kDa respectively and inhibited cell-free translation in rabbit reticulocyte lysate with an IC50 of 0.15 and 170 nM. The sequence of the first seven N-terminal amino acids in δ-momorcharin was DVNFGLA, which was different from the N-terminal sequences DVSFRLS and DVNFDLS for α- and β-momorcharins respectively.
- Subjects :
- chemistry.chemical_classification
Chromatography
Momordica
biology
Chemistry
Ribosome-inactivating protein
Ion chromatography
Bitter gourd
Fast protein liquid chromatography
Cell Biology
biology.organism_classification
Biochemistry
Amino acid
medicine.anatomical_structure
Affinity chromatography
Reticulocyte
medicine
Subjects
Details
- ISSN :
- 13572725
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- The International Journal of Biochemistry & Cell Biology
- Accession number :
- edsair.doi...........6519d62327be1273fee6cf5ac5b3029f