Linear SV Plot Ananlysis in Steady State and Transient State to Explore Fluorescence Quenching of Coumarin Derivative by Aniline

Static and transient state analysis related to fluorescence quenching of heterocyclic compound namely 3-Hydroxy-3-[2-oxo-2-(3-oxo-3H-benzo[f]chromen-2-yl)-ethyl]-1,3-dihydro-indol-2-one [3HBCD] in Toluene (nonpolar) and Butanol (polar protic) reveals the play of material diffusion in quenching mechanism. Time resolved method is used in Toluene a nonpolar solvent to further support the theory of material diffusion. Aniline is used as quencher to carry out the quenching studies. In both steady and transient state linear SV plots are observed with intercept ≈ 1. Correlation in the activation energy in both study state and transient state (Ea and E’a) suggests efficient quenching. Also calculated values of p (or p’) and the activation energies points that material diffusion has predominant role in quenching. This study help engineer the structure of new family of coumarin drugs with selective sensitivity which is of utmost importance in the field of chemistry and pharmaceutical industry.