Further Purification of a Macromolecular Hypotensive Substnace in Fomes pinicola

A macromolecular hypotensive substance in water extract of Fomes pinicola was purified by sequential fractionation on columns of Sephadex G-75, DEAE-cellulose, Sephadex G-150, Hydroxyapatite and Sepharose CL-4B gels. On the basis of its chemical properties and component analysis, it was considered to be a glycoprotein containing a large proportion of carbohydrate.