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Production of active oxygen and cytotoxicity of tumor cells on pulse laser photodynamic therapy

Authors :
T. Yamamoto
Haruyuki Minamitani
Akira Saito
T. Iino
K. Aizawa
T. Nagao
Source :
Proceedings of the 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 'Magnificent Milestones and Emerging Opportunities in Medical Engineering' (Cat. No.97CH36136).
Publication Year :
2002
Publisher :
IEEE, 2002.

Abstract

In this study, a doubled Q-switched Nd:YAG laser which has high peak power and lower thermal effect than that of a continuous wave laser, was employed to investigate the damage of tumor cells. As a photosensitizer, zinc coproporphyrin III (Zn CP-III) was prepared. Active oxygen from the photosensitizer was detected by method of luminol chemiluminescence at 430 nm-wavelength. Viability of human small cell carcinoma (MS-1) against the irradiation time, the concentration of Zn CP-III and the pulse energy was investigated. As the result, Zn CP-III produced active oxygen and cells treated by PDT showed a significant reduction of viability.

Details

Database :
OpenAIRE
Journal :
Proceedings of the 19th Annual International Conference of the IEEE Engineering in Medicine and Biology Society. 'Magnificent Milestones and Emerging Opportunities in Medical Engineering' (Cat. No.97CH36136)
Accession number :
edsair.doi...........63ff76c8b2d1ec42eacf3f792b0a2223