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Molecular typing of MRSA isolates by spa and PFGE
- Source :
- Journal of King Saud University - Science. 31:999-1004
- Publication Year :
- 2019
- Publisher :
- Elsevier BV, 2019.
-
Abstract
- Background Methicillin resistant S. aureus (MRSA) strains circulating among populations and crossing borders constitute a major problem for health control and require a fast and simple genotypic approach. Methods In the current study we compared staphylococcal protein A (spa) and pulsed field gel electrophoresis (PFGE) typing methods to genotype 106 MRSA clinical isolates. Results The genetic spectrum of the isolates was very diverse as revealed by the two typing approaches. In total, we identified 35 spa types in the study. The most frequently detected spa type in the study was t044 (30.18%), followed by t127 and t304 (5.6% each), t363 (4.6%), and t1200 and t002 (3.8% each). The rest of isolates were detected in low frequency and many were singletons. PFGE genotyping identified 34 pulsogroups. Most of the isolates were clustered in pulsogroup J. There was no clustering of the spa types into the pulsogroups. Conclusions MRSA isolates are very diverse in the region. In light of the observed MRSA diversity the spa typing could constitute a preferable approach for MRSA typing.
- Subjects :
- Multidisciplinary
Staphylococcal protein
02 engineering and technology
010501 environmental sciences
Biology
bacterial infections and mycoses
021001 nanoscience & nanotechnology
01 natural sciences
Microbiology
Molecular typing
Genotype
Pulsed-field gel electrophoresis
Typing
Typing methods
0210 nano-technology
Spa typing
Genotyping
0105 earth and related environmental sciences
Subjects
Details
- ISSN :
- 10183647
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Journal of King Saud University - Science
- Accession number :
- edsair.doi...........63fdda6f96b202b3938d8b20dca78ffb