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- Source :
- Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health. 5:62
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Objectives We evaluated role of sFlt-1 and VEGF in a preeclampsia model driven by RAS activation. We hypothesized as transgenic crossbreeds develop preeclampsia, features of hypertension, proteinuria, kidney and placenta end-organ damage will occur concurrently with (1) sFlt1 rising higher in preeclamptic mice, (2) VEGF remaining high in preeclamptic mice but equivalent to controls; and, (3) preeclamptic kidneys demonstrating increased VEGF binding. Methods Transgenic hAGT and hREN, or wild type (WT) mice were used. Non-gravid females underwent telemetric transmitter implantation for blood pressure (BP) measurements. Mice were cross-bred: hAGT × hREN for RAS preeclamptic model and pregnant controls (WT × WT). Blood and urine collected on days (GD) 12, 15, and 18. Vascular reactivity in mesenteric arteries investigated by wire myography using KCl, acetylcholine (ACH), phenylephrine (PE). ELISA performed on urine for albumin and on plasma for VEGF and sFlt-1. Necropsies were performed GD 18-19. Kidneys and placenta were sectioned for HE and immunostain. Results 11 RAS pregnancies and 9 WT pregnancies (WTP) compared. 12,414 h of BP data analyzed. RAS mice demonstrated higher BP and proteinuria. RAS mice had glomerular endotheliosis (80% vs. 11%; p = 0.02), and placental necrosis (60% vs. 0%; p Conclusions While RAS model of preeclampsia recapitulates human preeclamptic state with high fidelity, renal injury may actually be mediated by increased VEGF binding. Disclosures J.M. Denney: None. C.E. Shaw: None. A. Gendron-Fitzpatrick: None. I. Bird: None. D. Shah: None.
- Subjects :
- medicine.medical_specialty
Kidney
Proteinuria
business.industry
Obstetrics and Gynecology
medicine.disease
Pathophysiology
Preeclampsia
Endocrinology
medicine.anatomical_structure
Blood pressure
Placenta
Internal medicine
Internal Medicine
medicine
medicine.symptom
business
Phenylephrine
Mesenteric arteries
medicine.drug
Subjects
Details
- ISSN :
- 22107789
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health
- Accession number :
- edsair.doi...........62d7a5199263235846d26f7895cbc842