Synthesis of Duplex DNA Containing a Spin Labeled Analog of 2′ Deoxycytidine

We report the chemical synthesis of phosphoramidite 8, containing a spin labeled analog of deoxycytidine, C∗, and its incorporation into synthetic DNA. The EPR characteristics of the resulting DNAs indicated that the motion of the spin label was well-correlated with the uniform modes of the macromolecule, but that correlation of the spin label with internal motion was less effective than that achieved using a spin labeled quinolone, Q.