MOESM7 of New insights into the heterogeneity of Th17 subsets contributing to HIV-1 persistence during antiretroviral therapy

Additional file 7. Phenotypic stability versus plasticity of the four central memory CCR6+ T-cell subsets under Th17- or Th1-polarizing conditions. FACS-sorted CM subsets were cultured under Th17- and Th1-polarizing conditions for 14 days, as described in Fig. 5A, stained with CCR6, CCR4, and CXCR3Abs and analyzed by FACS. Shown are the statistical analyses for the expression of (A) CCR6 (B) CCR4, and (C) CXCR3, and the co-expression of (D) CCR6 and CCR4 (R6+R4+ phenotype) and (E) CCR6 and CXCR3 (R6+X3+ phenotype) on each subsets at day 0 (white bars) versus day 14 in culture under Th17- (black bars) and Th1-polarizing (grey bars) conditions. (A-E) The positivity gates where defined based on fluorescence minus one (FMO) controls. Results (mean ± SEM) were generated with matched subsets from n = 3 distinct subjects. Paired t-Test p-values are indicated on the graphs.