Investigation of HPV Infection and P53 Expression in Laryngeal Verrucous Carcinoma

ABS TRACT Objective: Ver ru co us car ci no ma is an un com mon, well-dif fe ren ti a ted va ri ant of squ a mo us cell car ci no ma that is re gar ded as a low gra de, lo cally in va si ve ma lig nant tu mor with ra re po ten ti al for dis tant me tas ta sis. Alt ho ugh the pat ho ge ne sis of ver ru co us car ci no ma is unk nown, many pos sib le ca u ses ha ve be en pro po sed. The le a ding the o ri es inc lu de hu man pa pil lo ma vi rus (HPV) in fec ti on and che mi cal car ci no ge ne sis. The aim of this study is to analy se the sta tus of the p53 ge ne and hu man pa pil lo ma vi rus (HPV) in fec ti on in ver ru co us car ci no ma of larynx, and to in ves ti ga te the cor re la ti on of p53 ex pres si on and HPV in fec ti on in ver ru co us car ci no ma of the larynx. Ma te ri al and Met hods: A ret ros pec ti ve study has be en per for med bet we en the ye ars of 1994 and 2004, com pri sing 21 con se cu ti ve pa ti ents with ver ru co us car ci no ma of larynx. De ta i led cli ni cal pa ra me ters ha ve al so be en re vi e wed. HPV iden ti fi ca ti on was ac comp lis hed with poly me ra se cha in re ac ti on. p53 im mu no his toc he mistry was per for med on 21 ver ru co us car ci no ma using poly clo nal se rum CM-1. Re sults: The HPV in fec ti on has be en de tec ted in in all of the twenty-one ca ses of ver ru co us car ci no mas. p53 im mu no his toc he mistry was per for med on 21 HPV con ta i ning ver ru co us car ci no mas and sho wed the ex pres si on of p53 in 14 ca ses. No sta tis ti cally sig ni fi cant dif fe ren ces we re fo und bet we en p53 ex pres si on and sta ge. Conc lu si on: The da ta in di ca te that HPV and the in cre a sed ex pres si on of p53 can co e xist in ver ru co us car ci no mas. Our study sug gests that verrucous car cinomas of the larynx may ari se thro ugh a still unk nown mec ha nism which ca u ses p53 inac ti va ti on or HPV in fec ti on.We think that p53 ove rex pres si on and on co ge nic HPV in fec ti on may co o pe ra te in the es tab lish ment of ma lig nant cells in ver ru co us car ci no ma of the larynx. The in te rac ti on bet we en the HPV in fec ti on and cer ta in cel lu lar on co ge nes such as p53 ne eds furt her studies.