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KINETICS OF β-[14<font>C</font>]CAROTENE IN A HUMAN SUBJECT USING ACCELERATOR MASS SPECTROMETRY
- Source :
- Isotope Production and Applications in the 21st Century.
- Publication Year :
- 2000
- Publisher :
- WORLD SCIENTIFIC, 2000.
-
Abstract
- {beta}-Carotene is a tetraterpenoid distributed widely throughout the plant kingdom. It is a member of a group of pigments referred to as carotenoids that have the distinction of serving as metabolic precursors to vitamin A in humans and many animals [1,2]. We used Accelerator Mass Spectrometry (AMS) [3] to determine the metabolic behavior of a physiologic oral dose of {beta}-[{sup 14}C]carotene (200 nanoCuries; 0.57 {micro}mol) in a healthy human subject. Serial blood specimens were collected for 210-d and complete urine and feces were collected for 17 and 10-d, respectively. Balance data indicated that the dose was 42% bioavailable. The absorbed {beta}-carotene was lost slowly via urine in accord with the slow body turnover of {beta}-carotene and vitamin A [4]. HPLC fractionation of plasma taken at early time points (0-24-h) showed the label was distributed between {beta}-carotene and retinyl esters (vitamin A) derived from intestinal metabolism.
Details
- Database :
- OpenAIRE
- Journal :
- Isotope Production and Applications in the 21st Century
- Accession number :
- edsair.doi...........61e3c174bdf6ec9012bb0e8dcd54550d