Complete Sequence of a Conjugative IncN Plasmid Harboring bla KPC-2 , bla SHV-12 , and qnrS1 from an Escherichia coli Sequence Type 648 Strain

We sequenced a novel conjugative bla KPC-2 -harboring IncN plasmid, pYD626E, from an Escherichia coli sequence type 648 strain previously identified in Pittsburgh, Pennsylvania. pYD626E was 72,800 bp long and carried four β-lactamase genes, bla KPC-2 , bla SHV-12 , bla LAP-1 , and bla TEM-1 . In addition, it harbored qnrS1 (fluoroquinolone resistance) and dfrA14 (trimethoprim resistance). The plasmid profile and clinical history supported the in vivo transfer of this plasmid between Klebsiella pneumoniae and Escherichia coli .