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Analysis of BRIP1 Variants among Korean Patients with BRCA1/2 Mutation-Negative High-Risk Breast Cancer
- Source :
- Cancer Research and Treatment. 48:955-961
- Publication Year :
- 2016
- Publisher :
- Korean Cancer Association, 2016.
-
Abstract
- Purpose The aim of the current study is to assess the spectrum of genetic variation in the BRIP1 gene among Korean high-risk breast cancer patients who tested negative for the BRCA1/2 mutation. Materials and methods Overall, 235 Korean patientswith BRCA1/2 mutation-negative high-risk breast cancerwere screened for BRIP1 mutations. The entire BRIP1 gene was analyzed using fluorescent-conformation sensitive gel electrophoresis. In silico analysis of BRIP1 variants was performed using PolyPhen-2 and SIFT. Results A total of 20 sequence alterations including 12 exonic and eight intronic variantswere found. Among the 12 exonic variants, 10 were missense and two were silent mutations. No protein-truncating mutation was found among the tested patients. Among the 10 missense variants, four (p.L263F, p.L340F, p.L474P, and p.R848H) were predicted to be pathogenic by both PolyPhen-2 and SIFT, and these variants were found in five patients. Of the four missense variants, p.L263F, p.L474P, and p.R848H localize to regions between the helicase motifs, while p.L340F resides in an iron-sulfur domain of BRIP1. Conclusion No protein-truncating mutation in BRIP1 was found among the tested patients. The contribution of BRIP1 variants is thought to be minor in Korean non-BRCA1/2 high-risk breast cancer.
- Subjects :
- 0301 basic medicine
Silent mutation
Oncology
Cancer Research
medicine.medical_specialty
business.industry
BRIP1
medicine.disease
BRCA2 Protein
03 medical and health sciences
Exon
030104 developmental biology
0302 clinical medicine
Breast cancer
030220 oncology & carcinogenesis
Internal medicine
Mutation (genetic algorithm)
Genetic variation
Medicine
Missense mutation
business
Subjects
Details
- ISSN :
- 20059256 and 15982998
- Volume :
- 48
- Database :
- OpenAIRE
- Journal :
- Cancer Research and Treatment
- Accession number :
- edsair.doi...........6197343e0099b595225b23371591551d