A highly selective synthesis of N-acetyllactosamine catalyzed by immobilised β-galactosidase from Bacillus circulans

Immobilisation of the β-galactosidase of Bacillus circulans on Eupergit C gave a highly selective and stable biocatalyst. The immobilised enzyme catalysed the transfer of the β-galactosyl residue of p-nitrophenyl β- d -galactopyranoside to N-acetylglucosamine with high selectivity for transfer to the 4-position of the glycosyl acceptor.