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Molecular typing of RhD-negative blood donors with C and/or E antigen
- Source :
- Scripta Medica. 50:98-102
- Publication Year :
- 2019
- Publisher :
- Centre for Evaluation in Education and Science (CEON/CEES), 2019.
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Abstract
- Background: Most people are either RhD positive or RhD negative, but there is also a number of persons with D antigen variants. The aim of this study was to prove, by using molecular diagnostic tests, whether the RHD gene and D antigen on the red cell membrane of the blood donors serologically typed as RhD-negative with RhD phenotype Ccddee and ccddEe, are so weak that they cannot be proven by serology techniques or the available anti-D test serums. Methods: Samples used are those of regular voluntary donors who were serotyped as RhD-negative, C and/or E positive. Samples were collected from voluntary donors at the Institute for Transfusion Medicine of the Republic of Srpska during the period from April 2016 to December 2018. Results: Among the serologically proven RhD-negative donors, 346 had C and/or E in their phenotype and those were subjected to molecular screening test. Conclusion: The first results of molecular typing match those published in literature, i.e. the RHD gene is present in some serologically RhD-negative forms, which was proven by molecular testing.
Details
- ISSN :
- 23037954 and 24903329
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Scripta Medica
- Accession number :
- edsair.doi...........6136b47953a78e692dbd09d3d561f115