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Multidimensional Electrophoresis of Proteins

Authors :
U. Kuck
R. Dernick
K.-J. Wiegers
Publication Year :
1985
Publisher :
Elsevier, 1985.

Abstract

Multidimensional electrophoresis (MD-analysis) is a technique for the high-resolution electrophoresis of proteins and polypeptides and for their characterization. It is presented here as three-dimensional electrophoresis for the separation and characterization of poliovirus-infected cells. This method is composed of three steps namely isoelectric focusing in urea-containing polyacrylamide gels (PAGIEF) as the first dimension, followed by electrophoresis of the gel piece from the first dimension in sodium dodecyl sulfate containing gels (SDS-PAGE) as the second dimension. Gel pieces from the two-dimensional gel subjected to limited proteolysis and subsequent SDS-PAGE of the peptides obtained constitute the third dimension. In addition, the influence of the most important parameters for the separation in the third dimension is demonstrated on serum albumins as substrates. The MD-analysis separates proteins and allows to characterize their relationship to each other and to identify unknown protein spots by comparison with peptide maps of known proteins.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........60ae986728bb6e5edabc2e47c8702568
Full Text :
https://doi.org/10.1016/b978-0-08-031739-7.50239-1