Additional file 1: of A unique insight into the MiRNA profile during genital chlamydial infection

Figure S1. Venn diagram of differentially expressed miRNAs after chlamydia infection. Figure S2. Venn diagram of differentially expressed miRNAs after chlamydia reinfection. Figure S3. Pathways predicted to be regulated by miRNAs differentially expressed in mice after chlamydia infection. Figure S4. Pathways predicted to be regulated by miRNAs differentially expressed in mice after chlamydia reinfection. Figure S5. A: Network One for Chlamydia infection shows CEBPA a transcription regulator as the focus molecule with associated miRNAs. B: Network Two for Chlam ydia infection shows CREB1 a transcription regulator as the focus molecule with associated miRNAs. C: Network One for Chlamydia reinfection shows RHOA, an enzyme, as the focus molecule with associated miRNAs and other molecules D: Network Two for Chlamydia reinfection shows mir-423-5p as the focus molecule with associated miRNAs and other molecules. E: Network Three for Chlamydia reinfection shows TNFSF10 as the focus molecule with associated miRNAs and other molecules. F: Network Four for Chlamydia reinfection shows the kinase SRC as the focus molecule with associated miRNAs and other molecules. G: Network Five for Chlamydia reinfection shows the transporter BCL2 as the focus molecule with associated miRNAs and other molecules. Table S1. Summary Analysis of Top Diseases and Biological Functions of miRNA expressed in the Chlamydia Infection and Re-Infection. Table S2. Networks for the Top Diseases and Biological Function Category of miRNA expressed in the Chlamydia Reinfection. Figure S6. Example of quality scoring for miRNA sequencing in this study. Figure S7. (A) Validation of miRNA 378b expression after single infection using qPCR, (B) Validation of miRNA 142-5p expression after single infection using qPCR. Figure S8. Comprehensive Analysis Pipeline for miRNA-seq data (CAP-miRSeq), adapted from Sun et al., 2014 [35]. (PDF 1710 kb)